version 7.0.4, r14, sp2 Search Results


sp 2  (ATCC)
96
ATCC sp 2
Sp 2, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+7%2E0%2E4%2C+r14%2C+sp2/Sp2/us08067664-1535-31-40
Average 96 stars, based on 1 article reviews
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sp 2 cells  (ATCC)
96
ATCC sp 2 cells
Sp 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+7%2E0%2E4%2C+r14%2C+sp2/Sp2/us08293245-149-33-38
Average 96 stars, based on 1 article reviews
sp 2 cells - by Bioz Stars, 2026-09
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sp2  (Proteintech)
93
Proteintech sp2
Sp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+7%2E0%2E4%2C+r14%2C+sp2/SP2+Antibody/pm40147441-1172-9-10
Average 93 stars, based on 1 article reviews
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neu5acα2-3galβ1-4glc-sp2-biotin  (GlycoTech Corporation)
90
GlycoTech Corporation neu5acα2-3galβ1-4glc-sp2-biotin
Neu5acα2 3galβ1 4glc Sp2 Biotin, supplied by GlycoTech Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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galβ1-4glc-sp2-biotin  (GlycoTech Corporation)
90
GlycoTech Corporation galβ1-4glc-sp2-biotin
Galβ1 4glc Sp2 Biotin, supplied by GlycoTech Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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ns3-pvax  (ImmunoGen Inc)
90
ImmunoGen Inc ns3-pvax
Ns3 Pvax, supplied by ImmunoGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ns3-pvax - by Bioz Stars, 2026-09
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sp2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sp2
FIG. 8. Electrophoresis mobility shift assay with GC box in the hsp47 promoter as a probe. A, 32P-labeled 35-bp oligonucleotides (hsp47-promoter) formed DNA-protein complexes in the extracts (4 mg of protein) prepared from BALB/c3T3 cells (lanes 4 and 5), F9 cells (lanes 6 and 7), HeLa cells (lanes 8 and 9), and 293 cells (lanes 10 and 11). Recombinant human Sp1 protein (0.2 mg) was similarly analyzed (lanes 2 and 3). Anti-Sp1 monoclonal antibody was added in lanes 3, 5, 7, 9, and 11. No extract or protein was added in lane 1. B, supershift analysis of GC box-binding protein by Sp1 family-specific antibodies. Extract was prepared from BALB/c3T3 cells. No extract was added in lane 1. Competition reactions were performed in the presence of a 100-fold molar excess of unlabeled oligonucleotides; lane 3, hsp47-pro- moter competitor; lane 4, putative Sp1-binding site-mutated hsp47 competitor (Sp1m);, and lane 5, consensus Sp1-binding site sequence competitor. Supershift analysis was performed in the presence of pre- immune serum (lane 6), and antibody against Sp1 (lane 7), <t>Sp2</t> (lane 8), Sp3 (lane 9), and Sp4 (lane 10). In lane 11, antibodies against Sp1, Sp2, Sp3, and Sp4 were mixed and added to the reaction.
Sp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+7%2E0%2E4%2C+r14%2C+sp2/Sp2+Antibody/pm10585450-70-3-10
Average 93 stars, based on 1 article reviews
sp2 - by Bioz Stars, 2026-09
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multibranched loop (mbl; mbl2)  (MBL Life science)
90
MBL Life science multibranched loop (mbl; mbl2)
FIG. 8. Electrophoresis mobility shift assay with GC box in the hsp47 promoter as a probe. A, 32P-labeled 35-bp oligonucleotides (hsp47-promoter) formed DNA-protein complexes in the extracts (4 mg of protein) prepared from BALB/c3T3 cells (lanes 4 and 5), F9 cells (lanes 6 and 7), HeLa cells (lanes 8 and 9), and 293 cells (lanes 10 and 11). Recombinant human Sp1 protein (0.2 mg) was similarly analyzed (lanes 2 and 3). Anti-Sp1 monoclonal antibody was added in lanes 3, 5, 7, 9, and 11. No extract or protein was added in lane 1. B, supershift analysis of GC box-binding protein by Sp1 family-specific antibodies. Extract was prepared from BALB/c3T3 cells. No extract was added in lane 1. Competition reactions were performed in the presence of a 100-fold molar excess of unlabeled oligonucleotides; lane 3, hsp47-pro- moter competitor; lane 4, putative Sp1-binding site-mutated hsp47 competitor (Sp1m);, and lane 5, consensus Sp1-binding site sequence competitor. Supershift analysis was performed in the presence of pre- immune serum (lane 6), and antibody against Sp1 (lane 7), <t>Sp2</t> (lane 8), Sp3 (lane 9), and Sp4 (lane 10). In lane 11, antibodies against Sp1, Sp2, Sp3, and Sp4 were mixed and added to the reaction.
Multibranched Loop (Mbl; Mbl2), supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+7%2E0%2E4%2C+r14%2C+sp2/mbl+2/pmc09869943-99-46-47
Average 90 stars, based on 1 article reviews
multibranched loop (mbl; mbl2) - by Bioz Stars, 2026-09
90/100 stars
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sp2  (DSMZ)
94
DSMZ sp2
FIG. 8. Electrophoresis mobility shift assay with GC box in the hsp47 promoter as a probe. A, 32P-labeled 35-bp oligonucleotides (hsp47-promoter) formed DNA-protein complexes in the extracts (4 mg of protein) prepared from BALB/c3T3 cells (lanes 4 and 5), F9 cells (lanes 6 and 7), HeLa cells (lanes 8 and 9), and 293 cells (lanes 10 and 11). Recombinant human Sp1 protein (0.2 mg) was similarly analyzed (lanes 2 and 3). Anti-Sp1 monoclonal antibody was added in lanes 3, 5, 7, 9, and 11. No extract or protein was added in lane 1. B, supershift analysis of GC box-binding protein by Sp1 family-specific antibodies. Extract was prepared from BALB/c3T3 cells. No extract was added in lane 1. Competition reactions were performed in the presence of a 100-fold molar excess of unlabeled oligonucleotides; lane 3, hsp47-pro- moter competitor; lane 4, putative Sp1-binding site-mutated hsp47 competitor (Sp1m);, and lane 5, consensus Sp1-binding site sequence competitor. Supershift analysis was performed in the presence of pre- immune serum (lane 6), and antibody against Sp1 (lane 7), <t>Sp2</t> (lane 8), Sp3 (lane 9), and Sp4 (lane 10). In lane 11, antibodies against Sp1, Sp2, Sp3, and Sp4 were mixed and added to the reaction.
Sp2, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+7%2E0%2E4%2C+r14%2C+sp2/SP2%2F0-AG14/us10717785-125-10-13
Average 94 stars, based on 1 article reviews
sp2 - by Bioz Stars, 2026-09
94/100 stars
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sp2 inspection tool  (KLA Tencor)
90
KLA Tencor sp2 inspection tool
FIG. 8. Electrophoresis mobility shift assay with GC box in the hsp47 promoter as a probe. A, 32P-labeled 35-bp oligonucleotides (hsp47-promoter) formed DNA-protein complexes in the extracts (4 mg of protein) prepared from BALB/c3T3 cells (lanes 4 and 5), F9 cells (lanes 6 and 7), HeLa cells (lanes 8 and 9), and 293 cells (lanes 10 and 11). Recombinant human Sp1 protein (0.2 mg) was similarly analyzed (lanes 2 and 3). Anti-Sp1 monoclonal antibody was added in lanes 3, 5, 7, 9, and 11. No extract or protein was added in lane 1. B, supershift analysis of GC box-binding protein by Sp1 family-specific antibodies. Extract was prepared from BALB/c3T3 cells. No extract was added in lane 1. Competition reactions were performed in the presence of a 100-fold molar excess of unlabeled oligonucleotides; lane 3, hsp47-pro- moter competitor; lane 4, putative Sp1-binding site-mutated hsp47 competitor (Sp1m);, and lane 5, consensus Sp1-binding site sequence competitor. Supershift analysis was performed in the presence of pre- immune serum (lane 6), and antibody against Sp1 (lane 7), <t>Sp2</t> (lane 8), Sp3 (lane 9), and Sp4 (lane 10). In lane 11, antibodies against Sp1, Sp2, Sp3, and Sp4 were mixed and added to the reaction.
Sp2 Inspection Tool, supplied by KLA Tencor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+7%2E0%2E4%2C+r14%2C+sp2/sp1++tool/us09230848-176-3-1
Average 90 stars, based on 1 article reviews
sp2 inspection tool - by Bioz Stars, 2026-09
90/100 stars
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immunogen ns3/4a-pvax  (ImmunoGen Inc)
90
ImmunoGen Inc immunogen ns3/4a-pvax
FIG. 8. Electrophoresis mobility shift assay with GC box in the hsp47 promoter as a probe. A, 32P-labeled 35-bp oligonucleotides (hsp47-promoter) formed DNA-protein complexes in the extracts (4 mg of protein) prepared from BALB/c3T3 cells (lanes 4 and 5), F9 cells (lanes 6 and 7), HeLa cells (lanes 8 and 9), and 293 cells (lanes 10 and 11). Recombinant human Sp1 protein (0.2 mg) was similarly analyzed (lanes 2 and 3). Anti-Sp1 monoclonal antibody was added in lanes 3, 5, 7, 9, and 11. No extract or protein was added in lane 1. B, supershift analysis of GC box-binding protein by Sp1 family-specific antibodies. Extract was prepared from BALB/c3T3 cells. No extract was added in lane 1. Competition reactions were performed in the presence of a 100-fold molar excess of unlabeled oligonucleotides; lane 3, hsp47-pro- moter competitor; lane 4, putative Sp1-binding site-mutated hsp47 competitor (Sp1m);, and lane 5, consensus Sp1-binding site sequence competitor. Supershift analysis was performed in the presence of pre- immune serum (lane 6), and antibody against Sp1 (lane 7), <t>Sp2</t> (lane 8), Sp3 (lane 9), and Sp4 (lane 10). In lane 11, antibodies against Sp1, Sp2, Sp3, and Sp4 were mixed and added to the reaction.
Immunogen Ns3/4a Pvax, supplied by ImmunoGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+7%2E0%2E4%2C+r14%2C+sp2/genetic+immunogen+containing+the+complete+ns3+4a+protease/us07439347-481-23-11
Average 90 stars, based on 1 article reviews
immunogen ns3/4a-pvax - by Bioz Stars, 2026-09
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sp2  (Medicago)
86
Medicago sp2
FIG. 8. Electrophoresis mobility shift assay with GC box in the hsp47 promoter as a probe. A, 32P-labeled 35-bp oligonucleotides (hsp47-promoter) formed DNA-protein complexes in the extracts (4 mg of protein) prepared from BALB/c3T3 cells (lanes 4 and 5), F9 cells (lanes 6 and 7), HeLa cells (lanes 8 and 9), and 293 cells (lanes 10 and 11). Recombinant human Sp1 protein (0.2 mg) was similarly analyzed (lanes 2 and 3). Anti-Sp1 monoclonal antibody was added in lanes 3, 5, 7, 9, and 11. No extract or protein was added in lane 1. B, supershift analysis of GC box-binding protein by Sp1 family-specific antibodies. Extract was prepared from BALB/c3T3 cells. No extract was added in lane 1. Competition reactions were performed in the presence of a 100-fold molar excess of unlabeled oligonucleotides; lane 3, hsp47-pro- moter competitor; lane 4, putative Sp1-binding site-mutated hsp47 competitor (Sp1m);, and lane 5, consensus Sp1-binding site sequence competitor. Supershift analysis was performed in the presence of pre- immune serum (lane 6), and antibody against Sp1 (lane 7), <t>Sp2</t> (lane 8), Sp3 (lane 9), and Sp4 (lane 10). In lane 11, antibodies against Sp1, Sp2, Sp3, and Sp4 were mixed and added to the reaction.
Sp2, supplied by Medicago, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+7%2E0%2E4%2C+r14%2C+sp2/sp2/10__32634_slash_0869___8155___2023___376___11___112___116-126-1-4
Average 86 stars, based on 1 article reviews
sp2 - by Bioz Stars, 2026-09
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Image Search Results


FIG. 8. Electrophoresis mobility shift assay with GC box in the hsp47 promoter as a probe. A, 32P-labeled 35-bp oligonucleotides (hsp47-promoter) formed DNA-protein complexes in the extracts (4 mg of protein) prepared from BALB/c3T3 cells (lanes 4 and 5), F9 cells (lanes 6 and 7), HeLa cells (lanes 8 and 9), and 293 cells (lanes 10 and 11). Recombinant human Sp1 protein (0.2 mg) was similarly analyzed (lanes 2 and 3). Anti-Sp1 monoclonal antibody was added in lanes 3, 5, 7, 9, and 11. No extract or protein was added in lane 1. B, supershift analysis of GC box-binding protein by Sp1 family-specific antibodies. Extract was prepared from BALB/c3T3 cells. No extract was added in lane 1. Competition reactions were performed in the presence of a 100-fold molar excess of unlabeled oligonucleotides; lane 3, hsp47-pro- moter competitor; lane 4, putative Sp1-binding site-mutated hsp47 competitor (Sp1m);, and lane 5, consensus Sp1-binding site sequence competitor. Supershift analysis was performed in the presence of pre- immune serum (lane 6), and antibody against Sp1 (lane 7), Sp2 (lane 8), Sp3 (lane 9), and Sp4 (lane 10). In lane 11, antibodies against Sp1, Sp2, Sp3, and Sp4 were mixed and added to the reaction.

Journal: The Journal of biological chemistry

Article Title: Separate cis-acting DNA elements control cell type- and tissue-specific expression of collagen binding molecular chaperone HSP47.

doi: 10.1074/jbc.274.50.35703

Figure Lengend Snippet: FIG. 8. Electrophoresis mobility shift assay with GC box in the hsp47 promoter as a probe. A, 32P-labeled 35-bp oligonucleotides (hsp47-promoter) formed DNA-protein complexes in the extracts (4 mg of protein) prepared from BALB/c3T3 cells (lanes 4 and 5), F9 cells (lanes 6 and 7), HeLa cells (lanes 8 and 9), and 293 cells (lanes 10 and 11). Recombinant human Sp1 protein (0.2 mg) was similarly analyzed (lanes 2 and 3). Anti-Sp1 monoclonal antibody was added in lanes 3, 5, 7, 9, and 11. No extract or protein was added in lane 1. B, supershift analysis of GC box-binding protein by Sp1 family-specific antibodies. Extract was prepared from BALB/c3T3 cells. No extract was added in lane 1. Competition reactions were performed in the presence of a 100-fold molar excess of unlabeled oligonucleotides; lane 3, hsp47-pro- moter competitor; lane 4, putative Sp1-binding site-mutated hsp47 competitor (Sp1m);, and lane 5, consensus Sp1-binding site sequence competitor. Supershift analysis was performed in the presence of pre- immune serum (lane 6), and antibody against Sp1 (lane 7), Sp2 (lane 8), Sp3 (lane 9), and Sp4 (lane 10). In lane 11, antibodies against Sp1, Sp2, Sp3, and Sp4 were mixed and added to the reaction.

Article Snippet: Antibodies against Sp1, Sp2, Sp3, and Sp4 were purchased from Santa Cruz Biotechnology.

Techniques: Electrophoresis, Mobility Shift, Labeling, Recombinant, Binding Assay, Sequencing